Journal:

Article Title: Defining Characteristics of Types I and II Apoptotic Cells in Response to TRAIL

doi:

Figure Lengend Snippet: Endogenous levels of FADD, BID, and procaspase 3 are higher in SW480 cells. Procaspase 8 and FLIP processing at the TRAIL-DISC. (A) Western analysis of the endogenous levels of the indicated proteins in NHH-2, NKNT-3, SW480 and HCT116 cells. (B) The cells were treated with His-TRAIL for 15 minutes (upper panel) or 30 minutes (lower panel) in the presence of the cross-linking antibody. The TRAIL-DISC complex was immunoprecipitated and tested for the presence of procaspase 8, FADD, and FLIP. “M” stands for mock, and “TR” or “T” for TRAIL treatment, as described in Materials and Methods.

Article Snippet: Cell Lines The human colon cancer cell line SW480 and the human lymphocytic cell line SKW6.4 were obtained from the ATCC (Manassas, VA).

Techniques: Western Blot, Immunoprecipitation

Journal:

Article Title: Defining Characteristics of Types I and II Apoptotic Cells in Response to TRAIL

doi:

Figure Lengend Snippet: Timing of BID, procaspase 3, and PARP cleavage in SW480 and HCT116 cells after TRAIL treatment. (A) The cells were treated with TRAIL in the presence of the different inhibitors for the indicated times — 4, 8, and 24 hours. The molecular composition of the different caspase inhibitors is given in Materials and Methods. BID cleavage was analyzed through Western analysis. (B) The cleavages of procaspase 3 and PARP were tested after 4, 8, and 24 hours of TRAIL treatment. Different caspase inhibitors were used to show the difference in their effects on procaspase 3 and PARP cleavage in HCT116 and SW480 cells. Caspases 9 and 2 inhibitors prevent procaspase 3 and PARP cleavages in HCT116 cells (see 8-hour time point). “M” stands for mock, and “T” or “Tr” for TRAIL treatment.

Article Snippet: Cell Lines The human colon cancer cell line SW480 and the human lymphocytic cell line SKW6.4 were obtained from the ATCC (Manassas, VA).

Techniques: Western Blot

Journal:

Article Title: Defining Characteristics of Types I and II Apoptotic Cells in Response to TRAIL

doi:

Figure Lengend Snippet: Cytochrome c release from mitochondria after TRAIL treatment in SW480 and HCT116 cells. Nuclear, heavy membrane, and cytosolic fractions were obtained after TRAIL treatment as described in Materials and Methods. The different fractions were analyzed for procaspase 8, BID, and cytochrome c. “M” stands for mock, and “Tr” for TRAIL treatment.

Article Snippet: Cell Lines The human colon cancer cell line SW480 and the human lymphocytic cell line SKW6.4 were obtained from the ATCC (Manassas, VA).

Techniques: Membrane

Journal: Oncotarget

Article Title: High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma

doi: 10.18632/oncotarget.18203

Figure Lengend Snippet: (A) The mRNA expression level of GPR116 in colon cancer and matched normal colon tissue derived from the Renji cohort was detected by real-time quantitative PCR. Error bars in the scatter plots represent SE. (B) Western blots showed GPR116 protein level in six paired colon cancer tissues (T) and adjacent normal colonic tissue (N) of patients from the Renji cohort and the relative GPR116 protein expression. GPR116 expression in Skrzypczak Colorectal (C) Skrzypczak Colorectal 2 (D) and Gaedcke Colorectal (E) grouped by normal colorectal tissue (1) and colorectal carcinoma (2) in Oncomine Cancer Microarray database. CRC: colorectal carcinoma; GPR116: G protein-coupled receptor 116; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; SE: standard error.

Article Snippet: Human colorectal carcinoma cell lines (HCT116, SW1116, SW480 and LOVO) and normal colonic mucosa epithelial cell line (FHC) were purchased from the American Type Culture Collection.

Techniques: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Western Blot, Microarray

Journal: Oncotarget

Article Title: High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma

doi: 10.18632/oncotarget.18203

Figure Lengend Snippet: Association between GPR116 expression and clinicopathologic features in patients with colorectal carcinoma

Article Snippet: Human colorectal carcinoma cell lines (HCT116, SW1116, SW480 and LOVO) and normal colonic mucosa epithelial cell line (FHC) were purchased from the American Type Culture Collection.

Techniques: Expressing

Journal: Oncotarget

Article Title: High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma

doi: 10.18632/oncotarget.18203

Figure Lengend Snippet: Univariate and multivariate analysis of prognostic parameters for survival in patients with colorectal carcinoma

Article Snippet: Human colorectal carcinoma cell lines (HCT116, SW1116, SW480 and LOVO) and normal colonic mucosa epithelial cell line (FHC) were purchased from the American Type Culture Collection.

Techniques: Expressing

Journal: Clinical Cancer Research

Article Title: Deregulated GSK3β Sustains Gastrointestinal Cancer Cells Survival by Modulating Human Telomerase Reverse Transcriptase and Telomerase

doi: 10.1158/1078-0432.ccr-09-0973

Figure Lengend Snippet: Fig. 1. Expression, phosphorylation, and activity of GSK3β in cell lines. A, protein extracts from HEK293 cells and colon (SW480, HCT116, HT-29), stomach (MKN-28, NKPS, TMK-1, NUGC-4), pancreatic (MIAPaCa-2, Capan-1, BxPC-3), and liver (HepG2) cancer cells were analyzed by Western immunoblotting for GSK3β expression and levels of pGSK3βS9, pGSK3βY216, and β-actin. B, GSK3β activity was detected by NRIKA (24) in HEK293 cells and the cancer cell lines indicated above. GSK3β was isolated by immunoprecipitation from 1 mg aliquot of cells lysate from each sample. An in vitro kinase reaction was then carried out in the presence of immunoprecipitated GSK3β, its substrate of recombinant human β-catenin protein (β-cateninHis), and nonradioisotopic ATP in the kinase reaction buffer. The resultant products were analyzed by Western immunoblotting for phosphorylation of β-cateninHis

Article Snippet: Human embryonic kidney cells (HEK293) and colon (SW480, HCT116, HT-29), pancreatic (MIAPaCa-2, Capan-1, BxPC-3) and liver (HepG2) cancer cell lines were obtained from the American Type Culture Collection.

Techniques: Expressing, Phospho-proteomics, Activity Assay, Western Blot, Isolation, Immunoprecipitation, In Vitro, Recombinant

Journal: Lab on a chip

Article Title: A microfluidic array with cellular valving for single cell co-culture.

doi: 10.1039/c0lc00172d

Figure Lengend Snippet: Fig. 6 Heterotypic single cell co-culture. Unlabelled single SW480 cells co-cultured with single SW480 cells metabolically labelled with the fluorescent product of calcein AM (A). A single cell couple (B), and the same couple immunostained with connexin 43 (red, C). A trap : cell diameter ratio of $2.0 was required for efficient (70%) cell coupling (D).

Article Snippet: Human SW480 epithelial, HT29 colon carcinoma and MCF-7 epithelial-like breast cancer cells were purchased from DSMZ (Germany).

Techniques: Co-Culture Assay, Cell Culture, Metabolic Labelling